Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.
Identifieur interne : 001811 ( Main/Exploration ); précédent : 001810; suivant : 001812Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.
Auteurs : Brad Gilbertson [Australie] ; Tian Zheng [Australie] ; Marie Gerber [France] ; Anne Printz-Schweigert [France] ; Chi Ong [Australie] ; Roland Marquet [France] ; Catherine Isel [France] ; Steven Rockman [Australie] ; Lorena Brown [Australie]Source :
- Viruses [ 1999-4915 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ARN viral, Protéines virales, Sialidase.
- métabolisme : ARN viral.
- physiologie : Virus de la grippe A.
- Analyse de mutations d'ADN, Animaux, Assemblage viral, Génétique inverse, Humains, Lignée cellulaire, Plasmides.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Neuraminidase, RNA, Viral, Viral Proteins.
- chemical , metabolism : RNA, Viral.
- physiology : Influenza A virus.
- Animals, Cell Line, DNA Mutational Analysis, Humans, Plasmids, Reverse Genetics, Virus Assembly.
Abstract
The influenza A virus genome comprises eight negative-sense viral RNAs (vRNAs) that form individual ribonucleoprotein (RNP) complexes. In order to incorporate a complete set of each of these vRNAs, the virus uses a selective packaging mechanism that facilitates co-packaging of specific gene segments but whose molecular basis is still not fully understood. Recently, we used a competitive transfection model where plasmids encoding the A/Puerto Rico/8/34 (PR8) and A/Udorn/307/72 (Udorn) PB1 gene segments were competed to show that the Udorn PB1 gene segment is preferentially co-packaged into progeny virions with the Udorn NA gene segment. Here we created chimeric PB1 genes combining both Udorn and PR8 PB1 sequences to further define the location within the Udorn PB1 gene that drives co-segregation of these genes and show that nucleotides 1776-2070 of the PB1 gene are crucial for preferential selection. In vitro assays examining specific interactions between Udorn NA vRNA and purified vRNAs transcribed from chimeric PB1 genes also supported the importance of this region in the PB1-NA interaction. Hence, this work identifies an association between viral genes that are co-selected during packaging. It also reveals a region potentially important in the RNP-RNP interactions within the supramolecular complex that is predicted to form prior to budding to allow one of each segment to be packaged in the viral progeny. Our study lays the foundation to understand the co-selection of specific genes, which may be critical to the emergence of new viruses with pandemic potential.
DOI: 10.3390/v8080238
PubMed: 27556479
PubMed Central: PMC4997600
Affiliations:
Links toward previous steps (curation, corpus...)
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A.schweigert@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region></placeName><orgName type="university">Université de Strasbourg</orgName></affiliation></author><author><name sortKey="Ong, Chi" sort="Ong, Chi" uniqKey="Ong C" first="Chi" last="Ong">Chi Ong</name><affiliation wicri:level="1"><nlm:affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia. Chi.Ong@seqirus.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Marquet, Roland" sort="Marquet, Roland" uniqKey="Marquet R" first="Roland" last="Marquet">Roland Marquet</name><affiliation wicri:level="4"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. r.marquet@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region></placeName><orgName type="university">Université de Strasbourg</orgName></affiliation></author><author><name sortKey="Isel, Catherine" sort="Isel, Catherine" uniqKey="Isel C" first="Catherine" last="Isel">Catherine Isel</name><affiliation wicri:level="4"><nlm:affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. c.isel@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region></placeName><orgName type="university">Université de Strasbourg</orgName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Unité de Génétique Moléculaire des Virus à ARN, Département de virologie, Institut Pasteur, Paris 75005, France. c.isel@ibmc-cnrs.unistra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Unité de Génétique Moléculaire des Virus à ARN, Département de virologie, Institut Pasteur, Paris 75005</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region></placeName></affiliation></author><author><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. Steve.Rockman@seqirus.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia. Steve.Rockman@seqirus.com.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Brown, Lorena" sort="Brown, Lorena" uniqKey="Brown L" first="Lorena" last="Brown">Lorena Brown</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. lorena@unimelb.edu.au.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author></analytic><series><title level="j">Viruses</title><idno type="eISSN">1999-4915</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Cell Line (MeSH)</term><term>DNA Mutational Analysis (MeSH)</term><term>Humans (MeSH)</term><term>Influenza A virus (physiology)</term><term>Neuraminidase (genetics)</term><term>Plasmids (MeSH)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>Reverse Genetics (MeSH)</term><term>Viral Proteins (genetics)</term><term>Virus Assembly (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>ARN viral (métabolisme)</term><term>Analyse de mutations d'ADN (MeSH)</term><term>Animaux (MeSH)</term><term>Assemblage viral (MeSH)</term><term>Génétique inverse (MeSH)</term><term>Humains (MeSH)</term><term>Lignée cellulaire (MeSH)</term><term>Plasmides (MeSH)</term><term>Protéines virales (génétique)</term><term>Sialidase (génétique)</term><term>Virus de la grippe A (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Neuraminidase</term><term>RNA, Viral</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Protéines virales</term><term>Sialidase</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>DNA Mutational Analysis</term><term>Humans</term><term>Plasmids</term><term>Reverse Genetics</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de mutations d'ADN</term><term>Animaux</term><term>Assemblage viral</term><term>Génétique inverse</term><term>Humains</term><term>Lignée cellulaire</term><term>Plasmides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The influenza A virus genome comprises eight negative-sense viral RNAs (vRNAs) that form individual ribonucleoprotein (RNP) complexes. In order to incorporate a complete set of each of these vRNAs, the virus uses a selective packaging mechanism that facilitates co-packaging of specific gene segments but whose molecular basis is still not fully understood. Recently, we used a competitive transfection model where plasmids encoding the A/Puerto Rico/8/34 (PR8) and A/Udorn/307/72 (Udorn) PB1 gene segments were competed to show that the Udorn PB1 gene segment is preferentially co-packaged into progeny virions with the Udorn NA gene segment. Here we created chimeric PB1 genes combining both Udorn and PR8 PB1 sequences to further define the location within the Udorn PB1 gene that drives co-segregation of these genes and show that nucleotides 1776-2070 of the PB1 gene are crucial for preferential selection. In vitro assays examining specific interactions between Udorn NA vRNA and purified vRNAs transcribed from chimeric PB1 genes also supported the importance of this region in the PB1-NA interaction. Hence, this work identifies an association between viral genes that are co-selected during packaging. It also reveals a region potentially important in the RNP-RNP interactions within the supramolecular complex that is predicted to form prior to budding to allow one of each segment to be packaged in the viral progeny. Our study lays the foundation to understand the co-selection of specific genes, which may be critical to the emergence of new viruses with pandemic potential.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27556479</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>26</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1999-4915</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>8</Issue><PubDate><Year>2016</Year><Month>08</Month><Day>20</Day></PubDate></JournalIssue><Title>Viruses</Title><ISOAbbreviation>Viruses</ISOAbbreviation></Journal><ArticleTitle>Influenza NA and PB1 Gene Segments Interact during the Formation of Viral Progeny: Localization of the Binding Region within the PB1 Gene.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.3390/v8080238</ELocationID><ELocationID EIdType="pii" ValidYN="Y">E238</ELocationID><Abstract><AbstractText>The influenza A virus genome comprises eight negative-sense viral RNAs (vRNAs) that form individual ribonucleoprotein (RNP) complexes. In order to incorporate a complete set of each of these vRNAs, the virus uses a selective packaging mechanism that facilitates co-packaging of specific gene segments but whose molecular basis is still not fully understood. Recently, we used a competitive transfection model where plasmids encoding the A/Puerto Rico/8/34 (PR8) and A/Udorn/307/72 (Udorn) PB1 gene segments were competed to show that the Udorn PB1 gene segment is preferentially co-packaged into progeny virions with the Udorn NA gene segment. Here we created chimeric PB1 genes combining both Udorn and PR8 PB1 sequences to further define the location within the Udorn PB1 gene that drives co-segregation of these genes and show that nucleotides 1776-2070 of the PB1 gene are crucial for preferential selection. In vitro assays examining specific interactions between Udorn NA vRNA and purified vRNAs transcribed from chimeric PB1 genes also supported the importance of this region in the PB1-NA interaction. Hence, this work identifies an association between viral genes that are co-selected during packaging. It also reveals a region potentially important in the RNP-RNP interactions within the supramolecular complex that is predicted to form prior to budding to allow one of each segment to be packaged in the viral progeny. Our study lays the foundation to understand the co-selection of specific genes, which may be critical to the emergence of new viruses with pandemic potential.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gilbertson</LastName><ForeName>Brad</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. bg@unimelb.edu.au.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Tian</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. tianchi.zheng@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerber</LastName><ForeName>Marie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. m.gerber@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Printz-Schweigert</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. A.schweigert@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ong</LastName><ForeName>Chi</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia. Chi.Ong@seqirus.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marquet</LastName><ForeName>Roland</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. r.marquet@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Isel</LastName><ForeName>Catherine</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Architecture et Réactivité de l'ARN, Université de Strasbourg, CNRS, IBMC, 15 rue René Descartes, Strasbourg 67084, France. c.isel@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Unité de Génétique Moléculaire des Virus à ARN, Département de virologie, Institut Pasteur, Paris 75005, France. c.isel@ibmc-cnrs.unistra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rockman</LastName><ForeName>Steven</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. Steve.Rockman@seqirus.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Seqirus, 63 Poplar Rd, Parkville 3052, Victoria, Australia. Steve.Rockman@seqirus.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brown</LastName><ForeName>Lorena</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute of Infection and Immunity, Parkville 3010, Victoria, Australia. lorena@unimelb.edu.au.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>08</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Viruses</MedlineTA><NlmUniqueID>101509722</NlmUniqueID><ISSNLinking>1999-4915</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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first="Chi" last="Ong">Chi Ong</name><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name><name sortKey="Rockman, Steven" sort="Rockman, Steven" uniqKey="Rockman S" first="Steven" last="Rockman">Steven Rockman</name><name sortKey="Zheng, Tian" sort="Zheng, Tian" uniqKey="Zheng T" first="Tian" last="Zheng">Tian Zheng</name></country><country name="France"><region name="Grand Est"><name sortKey="Gerber, Marie" sort="Gerber, Marie" uniqKey="Gerber M" first="Marie" last="Gerber">Marie Gerber</name></region><name sortKey="Isel, Catherine" sort="Isel, Catherine" uniqKey="Isel C" first="Catherine" last="Isel">Catherine Isel</name><name sortKey="Isel, Catherine" sort="Isel, Catherine" uniqKey="Isel C" first="Catherine" last="Isel">Catherine Isel</name><name sortKey="Marquet, Roland" sort="Marquet, Roland" uniqKey="Marquet R" first="Roland" last="Marquet">Roland Marquet</name><name sortKey="Printz Schweigert, Anne" sort="Printz Schweigert, Anne" uniqKey="Printz Schweigert A" first="Anne" last="Printz-Schweigert">Anne Printz-Schweigert</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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